Amount Used: ?1 Ug/106 cells BALB/c spleen cells were incubated with either PBS or rat IgM-FITC, following which small lymphocytes were gated and analyzed on a FACScan flow cytometer (BDIS, San Jose, CA).
To insure lot- to- lot consistency, each batch of product is tested to conform to characteristics of a standardreference reagent using immunofluorescence staining and analysis on a Becton Dickinson FACScanTM flowcytometer and/or ELISA. Representative data are included in this Technical Bulletin.
Warning
Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin,eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash withcopious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxichydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water before discardingto avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Since applications vary, each investigator should determine the optimum working dilutions of the product that is appropriate for their specific needs.
Other Applications Image 1 Data
Amount Used: ≤1 Ug/106 cells BALB/c spleen cells were incubated with either PBS or rat IgM-FITC, following which small lymphocytes were gated and analyzed on a FACScan flow cytometer (BDIS, San Jose, CA).
Storage
- The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mMborate buffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C - The fluorescein (FITC) and fluorescein- 5- maleimide (FLMA) conjugates are supplied as 0.5 mg in 1.0 mLPBS/NaN3. Store at 2- 8 C - The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C - The R- phycoerythrin (R- PE) and allophycocyanin (APC) conjugates are supplied as 0.1 mg in 1.0 mL ofPBS/NaN3 and a stabilizing agent. Store at 2- 8 C. Do not freeze! - The Spectral Red (SPRD) and R- phycoerythrin- Cyanine 7 (R- PE- CY 7) conjugates are supplied as0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2- 8 C. Do not freeze! - The low endotoxin, azide- free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL ofPBS. Aliquot and store at or below - 20 C - With the exception of the low endotoxin, azide- free product, the vials should be stored at 2- 8 C Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Aliquotand freeze the low endotoxin, azide- free product at - 20 C immediately upon receipt. Each reagent isstable for the period shown on the bottle label if stored as directed.
Gene Full Name
Ig mu chain C region
What is the species homology for "Rat IgM Antibody : APC (OASB00138)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "".
How long will it take to receive "Rat IgM Antibody : APC (OASB00138)"?
This item is available "Domestic: within 1-2 weeks delivery | International: 1-2 weeks".
What buffer format is "Rat IgM Antibody : APC (OASB00138)" provided in?
This item is provided in "". Additional format options may be available. For more information please contact info@avivasysbio.com.
What are other names for "Rat IgM Antibody : APC (OASB00138)"?
This target may also be called "" in publications.
What is the shipping cost for "Rat IgM Antibody : APC (OASB00138)"?
The shipping cost for this item is within the US. Please contact us for specific shipping for international orders.
What is the guarantee for "Rat IgM Antibody : APC (OASB00138)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
Can I get bulk pricing for "Rat IgM Antibody : APC (OASB00138)"?
You can get bulk pricing for this item by going here.
What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "". Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics.
What protocols are available for "Rat IgM Antibody : APC (OASB00138)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
What are positive controls for ""?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
What are negative controls for ""?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
What other proteins interact with ""?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
What biological processes are associated with ""?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
What cellular components are associated with ""?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
What protein functions are associated with ""?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.
IGHM Antibody - N-terminal region (OAAB04516)
Catalog #: OAAB04516
Application: WB, IHC
Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
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