ELISA: 1:80,000, IHC: 1:1,000 - 1:5,000, WB: 1:1,000 to 1:10,000
Form/Appearance
Liquid (sterile filtered)
Purity
Anti-RAT IgM (mu chain) (GOAT) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rat IgM and Rat Serum. No reaction was observed against other rat heavy or light chain proteins.
Conjugation
Unconjugated
Storage
Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
Buffer/Preservatives
0.01% (w/v) Sodium Azide
Alternative names
Goat anti-rat IgM
Note
For research use only
Application notes
Anti-RAT IgM (mu chain) (GOAT) Antibody has been tested by ELISA and western blot and is assayed against Rat IgM in a standard capture ELISA using Peroxidase Conjugated Streptavidin. A working dilution of 1:15,000 to 1:70,000 of the concentration is suggested for this product. Rat IgM antibody is suitable for immunoassays including western blot and ELISA.