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> Rat IgM (mu chain) antibody (Alkaline Phosphatase)

Rat IgM (mu chain) antibody (Alkaline Phosphatase)

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Conditionnement : 1mg

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Rat IgM (mu chain) antibody (Alkaline Phosphatase)

Rat IgM (mu chain) antibody (Alkaline Phosphatase)

Catalog Number: orb347817

Catalog Numberorb347817
CategoryAntibodies
DescriptionRat IgM (mu chain) antibody (Alkaline Phosphatase)
Species/HostGoat
ClonalityPolyclonal
Tested applicationsDOT, ELISA, IHC, WB
ReactivityRat
IsotypeIgG
ImmunogenRat IgM whole molecule
Concentration1.0 mg/mL
Dilution rangeELISA: 1:10,000 - 1:30,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500
Form/AppearanceLiquid (sterile filtered)
PurityThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Rat IgM and Rat Serum. No reaction was observed against other rat heavy or light chain proteins.
ConjugationAP
StorageStore vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Buffer/Preservatives0.01% (w/v) Sodium Azide
Alternative namesgoat anti-Rat IgM (mu chain) Antibody Alkaline Pho
NoteFor research use only
Application notesAnti-Rat IgM Alkaline Phosphatase antibody has been tested by ELISA and dot blot and is assayed against 1.0 ug of Rat IgM in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1:500 to 1:2,000 is suggested for this product.
Expiration Date12 months from date of receipt.