General Info
| Applications: | CLIA |
| Reactivity: | Rat |
| Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: | 0.19ng/mL |
| Detection Limit: | 0.31~20ng/mL |
| Short Description: | This rat S100A11 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: | 3.5h |
| Detection: | Chemiluminescence |
Information
| Gene Symbol: | S100a11 |
| Gene ID: | 445415 |
| Uniprot ID: | S10AB_RAT |
| Specificity: | This kit recognizes Rat S100A11 in samples. No significant cross-reactivity or interference between Rat S100A11 and analogues was observed. |
| Sample Type: | Serum, plasma and other biological fluids |
Description
| Post Translational Modifications | Phosphorylation at Thr-5 significantly suppresses homodimerization and promotes association with NCL/nucleolin which induces nuclear translocation. |
| Function | Facilitates the differentiation and the cornification of keratinocytes. |
| Protein Name | Protein S100-A11CalgizzarinS100 Calcium-Binding Protein A11 |
| Database Links | Reactome: R-RNO-6798695 |
| Cellular Localisation | CytoplasmNucleus |
| Alternative CLIA Names | Protein S100-A11 CLIA kitCalgizzarin CLIA kitS100 Calcium-Binding Protein A11 CLIA kitS100a11 CLIA kit |
| Specificity | This kit recognizes Rat S100A11 in samples. No significant cross-reactivity or interference between Rat S100A11 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
|---|---|---|
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 99-114 | 105 |
| EDTA plasma(n=8) | 93-108 | 100 |
| Cell culture media(n=8) | 90-105 | 96 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
|---|---|---|---|---|---|---|
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 1.11 | 2.11 | 6.84 | 1.18 | 1.98 | 7.41 |
| Standard deviation | 0.12 | 0.22 | 0.42 | 0.15 | 0.20 | 0.45 |
| CV (%) | 10.81 | 10.43 | 6.14 | 12.71 | 10.10 | 6.07 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance