Rho A Antibody (26C4) is an IgG₁ κ mouse monoclonal Rho A antibody (also designated RHOA antibody) that detects the Rho A protein of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM. Rho A Antibody (26C4) is available as both the non-conjugated anti-Rho A antibody form, as well as multiple conjugated forms of anti-Rho A antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor^® conjugates. The Ras p21 family of guanine nucleotide proteins has been widely studied in view of its apparent role in signal transduction pathways and high frequency of mutations in human malignancies. It is now clear, however, that the Ras proteins (H-, K- and N-Ras p21) are members of a much larger superfamily of related proteins. Six members of this family, Rap 1A, Rap 1B, Rap 2, R-Ras, Ral A and Ral B, exhibit approximately 50% amino acid homology to Ras. The six mammalian Rho proteins (Rho A, B, C, G, 7 and 8) are approximately 30% homologous to Ras and are expressed in a wide range of cell types. Both Ras p21 and Rho p21, as well as other members of the Ras superfamily, contain a carboxy-terminal CAAX sequence (C, cysteine; A, aliphatic amino acid; X, any amino acid) which in the case of Ras has been shown to be essential for correct localization and function.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

References:

1. Post-translational modifications of p21rho proteins.  |  Adamson, P., et al. 1992. J Biol Chem. 267: 20033-8. PMID: 1400319
2. Expression of the ras-related ralA, rho12 and rab genes in adult mouse tissues.  |  Olofsson, B., et al. 1988. Oncogene. 3: 231-4. PMID: 2457862
3. Identification of distinct cytoplasmic targets for ras/R-ras and rho regulatory proteins.  |  Garrett, MD., et al. 1989. J Biol Chem. 264: 10-3. PMID: 2491843
4. Scrape-loading of Swiss 3T3 cells with ras protein rapidly activates protein kinase C in the absence of phosphoinositide hydrolysis.  |  Morris, JD., et al. 1989. Oncogene. 4: 27-31. PMID: 2536916
5. The ras superfamily proteins.  |  Chardin, P. 1988. Biochimie. 70: 865-8. PMID: 3145021
6. ras genes.  |  Barbacid, M. 1987. Annu Rev Biochem. 56: 779-827. PMID: 3304147
7. Nucleotide sequence of human rho cDNA clone 12.  |  Yeramian, P., et al. 1987. Nucleic Acids Res. 15: 1869. PMID: 3822842
8. A novel ras-related gene family.  |  Madaule, P. and Axel, R. 1985. Cell. 41: 31-40. PMID: 3888408

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-RhoA Antibody (26C4) has 1,281 citations in a variety of scientific publications.

Primary antibodies like Anti-RhoA Antibody (26C4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.