RPS14 - Ribosomal protein S14 (mitochondrial)
Référence AS164094
Conditionnement : 50µg
Marque : Agrisera
Product no: AS16 4094
AS16 4094 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
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Immunogen: Contact us - Application Examples
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Application example
30 µg of mitochondrial fraction from Arabidopsis thaliana extracted with buffer (sucrose, tetrasodiumpyrophosphate, EDTA, KH2O4, PVP-40, BSA, pH 7.5) and denatured with 56°C for 15 min at 650 rpm were separated on 12 % SDS-PAGE and blotted overnight (12V) to PVDF using wet transfer. Blots were blocked with 5% skimmed powder milk in TBS-T for 1h at room temperature (RT) with agitation. Blot was incubated in the purified anti S14 serum at a dilution of 1: 1 000 for 1,5h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera,Vous serez peut-être également intéressé par les produits suivants :
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