ASM10535-STR-100 | Rubicon Antibody Hexabiogen

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Rubicon Polyclonal Antibody (ASM10535). Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-Rubicon Polyclonal Antibody (ASM10535) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Late Endosome, Lysosome, Early Endosome . Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Rubicon Antibody. (D) Composite.
Western blot analysis of Human HeLa cell lysates showing detection of ~108 kDa Rubicon protein using Rabbit Anti-Rubicon Polyclonal Antibody (ASM10535). Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Rubicon Polyclonal Antibody (ASM10535) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~108 kDa.

Product Information
Application Applications Legend: WB=Western Blot IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin-embedded Sections) IHC-F=Immunohistochemistry (Frozen Sections) IF=Immunofluorescence FC=Flow Cytopmetry IC=Immunochemistry ICC=Immunocytochemistry E=ELISA IP=Immunoprecipitation DB=Dot Blot CHIP=Chromatin Immunoprecipitation FA=Fluorescence Assay IEM=Immunoelectronmicroscopy EIA=Enzyme Immunoassay	WB, ICC
Primary Accession	Q92622
Other Accession	NP_001139114.1
Host	Rabbit
Reactivity	Human
Clonality	Polyclonal
Description	Rabbit Anti-Human Rubicon Polyclonal
Target/Specificity	Detects ~95 kDa.
Other Names	Run domain Beclin-1-interacting and cysteine-rich domain-containing protein Antibody, KIAA0226 Antibody, Rubicon Antibody, Baron Antibody, RUBCN Antibody, Beclin-1 associated RUN domain containing protein Antibody
Immunogen	Synthetic peptide from the N-terminal of Human Rubicon
Purification	Peptide Affinity Purified
Storage	-20ºC
Storage Buffer	PBS, 50% glycerol, 0.09% sodium azide
Shipping Temperature	Blue Ice or 4ºC
Certificate of Analysis	A 1:1000 dilution of SPC-668 was sufficient for detection of Rubicon in 15 µg of human Hela cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization	Late Endosome \| Lysosome \| Early Endosome

Rubicon Antibody