Réactifs et instruments pour l'immunologie, la biologie cellulaire et la biologie moléculaire.
 
> SHANK1 Antibody

SHANK1 Antibody

Imprimer Imprimer
Identifiez-vous

Référence ASM10205-P594-100

Conditionnement : Onrequest

Marque : Abcepta

Contactez votre distributeur local :


Téléphone : +1 850 650 7790

 
  • IHC - SHANK1 Antibody ASM10205
    Immunohistochemistry analysis using Mouse Anti-SHANK1 Monoclonal Antibody, Clone S22-21 (ASM10205). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-SHANK1 Monoclonal Antibody (ASM10205) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Filaggrin-like staining (upper layer aggregations of staining).
  • WB - SHANK1 Antibody ASM10205
    Western Blot analysis of Rat brain membrane lysate showing detection of SHANK1 protein using Mouse Anti-SHANK1 Monoclonal Antibody, Clone S22-21 (ASM10205). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-SHANK1 Monoclonal Antibody (ASM10205) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC
Primary Accession Q9WV48
Other Accession NP_113939.2
Host Mouse
Isotype IgG1
Reactivity Human, Mouse, Rat
Clonality Monoclonal
Description Mouse Anti-Rat SHANK1 Monoclonal IgG1
Target/Specificity Detects ~190-220 kDa (alternative splice variants). No cross-reactivity against Shank2 or Shank3.
Other Names GKAP/SAPAP interacting protein Antibody, OTTHUMP00000174437 Antibody, SH3 and multiple ankyrin repeat domains 1 Antibody, SH3 and multiple ankyrin repeat domains protein 1 Antibody, SH3/ankyrin domain gene 1 Antibody, SHAN1_HUMAN Antibody, SHANK 1 Antibody, Shank1 Antibody, Shank1a Antibody, Somatostatin receptor interacting protein Antibody, Somatostatin receptor-interacting protein Antibody, SPANK 1 Antibody, SPANK1 Antibody, SSTR interacting protein Antibody, SSTR-interacting protein Antibody, SSTRIP Antibody, Synamon Antibody
Clone Names S22-21
Immunogen Fusion protein amino acids 469-691 (SH3/PDZ domains) of rat Shank1
Purification Protein G Purified
Storage -20ºC
Storage Buffer PBS pH7.4, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis 1 µg/ml of SMC-329 was sufficient for detection of Shank1 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Cellular Localization Cytoplasm | Cell Junction | Synapse | Postsynaptic Cell Membrane | Postsynaptic Density