Stat6 Antibody (D-1) is an IgG_2b κ mouse monoclonal Stat6 antibody (also designated IL-4 Stat antibody) that detects the Stat6 protein of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. Stat6 Antibody (D-1) is available as both the non-conjugated anti-Stat6 antibody form, as well as multiple conjugated forms of anti-Stat6 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor^® conjugates. Membrane receptor signaling by various ligands, including interferons and growth hormones such as EGF, induces activation of JAK kinases which then leads to tyrosine phosphorylation of proteins that have been designated Stats (signal transducers and activators of transcription. The first members of this family to be described include Stat1α p91, Stat1β p84 (a form of p91 that lacks 38 COOH-terminal amino acids) and Stat2 p113. Stat1 and Stat2 are induced by IFN-a and form a heterodimer which is part of the ISGF3 transcription factor complex. Stat3, which becomes activated in response to epidermal growth factor (EGF) and interleukin-6 (IL-6), but not interferon-γ (IFN-γ) or Stat4, is an additional member of this family. It has been suggested that the phosphorylated forms of both Stat3 and Stat4 form homodimers as well as heterodimers with the other members of the Stat family, and that differential activation of different Stat proteins in response to different ligands should help to explain specificity in nuclear signaling from the cell surface. Highest expression of Stat4 is seen in testis and myeloid cells. IL-12 has been identified as an activator of Stat4. Other members of the Stat family include Stat5, which has been shown to be activated by prolactin and by IL-3, and Stat6 (also designated IL-4 Stat), which is involved in IL-4-activated signaling pathways.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

References:

1. Tyrosine-phosphorylated Stat1 and Stat2 plus a 48-kDa protein all contact DNA in forming interferon-stimulated-gene factor 3.  |  Qureshi, SA., et al. 1995. Proc Natl Acad Sci U S A. 92: 3829-33. PMID: 7537377
2. Cooperative transcriptional activity of Jun and Stat3 beta, a short form of Stat3.  |  Schaefer, TS., et al. 1995. Proc Natl Acad Sci U S A. 92: 9097-101. PMID: 7568080
3. Transcriptional responses to polypeptide ligands: the JAK-STAT pathway.  |  Schindler, C. and Darnell, JE. 1995. Annu Rev Biochem. 64: 621-51. PMID: 7574495
4. Interleukin-3, erythropoietin, and prolactin activate a STAT5-like factor in lymphoid cells.  |  Pallard, C., et al. 1995. J Biol Chem. 270: 15942-5. PMID: 7608147
5. Stat4, a novel gamma interferon activation site-binding protein expressed in early myeloid differentiation.  |  Yamamoto, K., et al. 1994. Mol Cell Biol. 14: 4342-9. PMID: 8007943
6. An interleukin-4-induced transcription factor: IL-4 Stat.  |  Hou, J., et al. 1994. Science. 265: 1701-6. PMID: 8085155
7. Stat3: a STAT family member activated by tyrosine phosphorylation in response to epidermal growth factor and interleukin-6.  |  Zhong, Z., et al. 1994. Science. 264: 95-8. PMID: 8140422
8. Jak-STAT pathways and transcriptional activation in response to IFNs and other extracellular signaling proteins.  |  Darnell, JE., et al. 1994. Science. 264: 1415-21. PMID: 8197455

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Stat6 Antibody (D-1) has 40 citations in a variety of scientific publications.

Primary antibodies like Anti-Stat6 Antibody (D-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

L'anticorps p-Stat6 préféré est l'Anticorps p-Stat6 (pY641.18): sc-136019.