SV40 T Antigen Cell Immortalization Kit
Référence CILV01
Conditionnement : 1Kit
Marque : Alstem
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SV40 T Antigen Cell Immortalization Kit
Description:
It is well known that primary cells only undergo a pre-determined and finite number of cell divisions in culture. After limited population doublings (the number of which varies by species, cell type, and culture conditions), primary cells enter a state, so-called replicative senescence, where they can no longer divide. To surpass senescence, several methods exist for immortalizing mammalian cells in culture. Simian virus 40 (SV40) T antigen has been n to be the simplest and most reliable agent for the immortalization of many different cell types. Recent studies have also n that SV40 T antigen can induce Telomerase activity in the infected cells.
Specifications:
| Product Name | SV40 T Antigen Cell Immortalization Kit |
|---|---|
| Shipping Condition | Dry Ice - Overnight Shipping |
| Storage and Stability | Store at -80 °C (stable up to 6 months) |
| Quality Control | The packaged lentivector constructs are provided as frozen VSV-G pseudotyped viral particles. The titer of the lentivirus is measured by Clontech's Lenti-X qRT-PCR Titration Kit (Cat. # 631235). Each lot of Cell Immortalization Kit is functionally tested in fibroblasts. |
| Restricted Use | For Research Use Only. Not for use in diagnostic or therapeutic procedures. |
Benischke AS et al., Sci Rep. 2017 Jul 27;7(1):6656. doi: 10.1038/s41598-017-06523-2.