Swine IgG Human IgE antibody
Référence orb21750-10mg
Conditionnement : 10mg
Marque : Biorbyt
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Swine IgG Human IgE antibody
Catalog Number: orb21750
| Catalog Number | orb21750 |
|---|---|
| Category | Antibodies |
| Description | Purified IgG fraction of polyclonal swine antiserum to Human IgE, Fc specific. As unlabelled secondary antibody for indirect detection of IgE in human cell, tissue substrates and body fluids in immunofluorescence and immunoenzyme assay methods; for the production of immunoconjugates with a selected marker; to prepare insoluble immunoaffinity adsorbents by coupling to an artificial carrier; as catching or detecting antibody reagent in non-isotopic assay methodology (e.g. ELISA) to identify and measure IgE in human serum or other body fluid. When applied in any immunocytochemical or histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established. Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1:250; in ELISA and comparable non-precipitating antibody-binding assays between 1:500 and 1:5,000. |
| Species/Host | Porcine |
| Clonality | Polyclonal |
| Form/Appearance | Purified hyperimmune swine IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity. |
| Conjugation | Unconjugated |
| Hazard Information | This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information. |
| Source | Purified homogenous IgE isolated from human serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
| Storage | Storage: The lyophilized IgG fraction is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product |
| Buffer/Preservatives | Purified hyperimmune swine IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity. |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |