TAGLN2 ELISA Kit (Human)

Référence OKEH00446

Conditionnement : 96Wells

Marque : Aviva Systems Biology

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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetPrintable COA datasheet for OKEH00446
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology TAGLN2 ELISA Kit (Human) (OKEH00446) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Human TAGLN2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing,a biotinylated detector antibody specific for Human TAGLN2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Human TAGLN2 captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 81%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.7% (n = 20)
Mean Inter-assay CV%: < 6.9% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-TAGLN2 Microplate96 Wells (12 x 8 Well strips)
TAGLN2 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated TAGLN21 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity0.078 ng/mL
SpecificityNatural and recombinant Human Transgelin-2
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolTAGLN2
Gene Full Nametransgelin 2
Alias Symbolsepididymis secretory sperm binding protein;epididymis tissue protein Li 7e;HA1756;SM22-alpha homolog;transgelin-2.
NCBI Gene Id8407
Protein NameP37802
Description of TargetTransgelin-2
Uniprot IDhttps://www.uniprot.org/uniprot/P37802
Protein Accession #NP_001264152.1
Nucleotide Accession #NM_001277223.1
Protein Size (# AA)199
Molecular Weight22 kDa

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