- More Files
Fluorescent In Situ Hybridization (Formalin/PFA-fixed paraffin-embedded sections)
human ovary cancer (FFPE) stained with TERT/CEN5q FISH Probe . human ovary cancer showed TERT amplification.
Hybridization position of the probes on the chromosome:
Hybridization position of the probes on the chromosome:
- Specification
Product Description
Labeled FISH probes for identification of gene amplification using Fluorescent In Situ Hybridization Technique. ().
Probe 1
Name: TERT
Size: Approximately 410kb
Fluorophore: Tex Red
Location: 5p15.33Probe 2
Name: CEN5q
Size: Approximately 700kb
Fluorophore: FITC
Location: 5q11.2Probe Gap
The gap between two probes is approximately 50,500 kb.
Origin
Human
Source
Genomic DNA
Reactivity
Human
Form
Liquid
Notice
We strongly recommend the customer to use FFPE FISH PreTreatment Kit 1 (Catalog #: KA2375 or KA2691) for the pretreatment of Formalin-Fixed Paraffin-Embedded (FFPE) tissue sections.
Regulation Status
For research use only (RUO)
Quality Control Testing
Representative images of normal human cell (lymphocyte) stain with the dual color FISH probe. The left image is chromosomes at metaphase, and the right image is an interphase nucleus.
Supplied Product
DAPI Counterstain (1500 ng/mL ) 125 uL for each 100 uL FISH Probe
Storage Instruction
Store at 4°C in the dark.
Note
Hybridization position of the probes on the chromosome:
- Applications
Fluorescent In Situ Hybridization (Cell)
Fluorescent In Situ Hybridization (Formalin/PFA-fixed paraffin-embedded sections)
human ovary cancer (FFPE) stained with TERT/CEN5q FISH Probe . human ovary cancer showed TERT amplification. - Gene Info — TERT
Entrez GeneID
7015Gene Name
TERT
Gene Alias
EST2, TCS1, TP2, TRT, hEST2
Gene Description
telomerase reverse transcriptase
Gene Ontology
HyperlinkGene Summary
Telomerase is a ribonucleoprotein polymerase that maintains telomere ends by addition of the telomere repeat TTAGGG. The enzyme consists of a protein component with reverse transcriptase activity, encoded by this gene, and an RNA component which serves as a template for the telomere repeat. Telomerase expression plays a role in cellular senescence, as it is normally repressed in postnatal somatic cells resulting in progressive shortening of telomeres. Deregulation of telomerase expression in somatic cells may be involved in oncogenesis. Studies in mouse suggest that telomerase also participates in chromosomal repair, since de novo synthesis of telomere repeats may occur at double-stranded breaks. Alternatively spliced variants encoding different isoforms of telomerase reverse transcriptase have been identified; the full-length sequence of some variants has not been determined. Alternative splicing at this locus is thought to be one mechanism of regulation of telomerase activity. [provided by RefSeq
Other Designations
telomerase catalytic subunit
- Interactome
- Disease