Tris Buffered Saline with Tween 20: 1 L of 20X
Référence sc-281695
Conditionnement : 1L
Marque : Santa Cruz Biotechnology
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Tris Buffered Saline with Tween 20: 1 L of 20X: sc-281695
Tris Buffered Saline with Tween 20: 1 L of 20X
Noms alternatifs:
20X TBST
Application:
Tris Buffered Saline with Tween 20: 1 L of 20X is a convenient prepared buffer for use in various applications
Pour la Recherche Uniquement. Non conforme pour le Diagnostic ou pour une Utilisation Thérapeutique.
* Consulter le Certificat d'Analyses pour les données spécifiques à un lot (incluant la teneur en eau).
ACCÈS RAPIDE AUX LIENS
Informations pour la commande
Références bibliographiques
Description
Information Technique
Données de Sécurité
SDS & Certificat d’Analyses
Tris Buffered Saline with Tween 20: 1 L of 20X is a high-quality 20X concentrate for dilution to a 1X working solution commonly used as a wash solution for western blot membranes and microtiter plate wells in ELISA assays. Tris Buffered Saline with Tween 20 (TBST) is an optimal formulation of pH stabilizers, salts and detergents designed to effectively remove excess material from the microtiter plate wells without disrupting the ELISA binding reaction. By maintaining the proper buffering environment, unbound components can be washed away without suppressing antigen-antibody binding interactions, thereby reducing nonspecific background and increasing the specific signal.
Additional sizes and formats available:
Tris Buffered Saline with Tween 20: 1 L of 1X (sc-362187)
Tris Buffered Saline with Tween 20: 100 ml of 10X (sc-362309)
Tris Buffered Saline with Tween 20: 500 ml of 10X (sc-362310)
Tris Buffered Saline with Tween 20: 1 L of 10X (sc-24953)
Tris Buffered Saline with Tween 20: 1 L of 20X (sc-281695)
Tris Buffered Saline with Tween 20: 5 L of 20X (sc-362311)
Tris Buffered Saline with Tween 20: powder for 100 ml of 10X (sc-362190)
Tris Buffered Saline with Tween 20: powder for 500 ml of 10X (sc-362312)
Tris Buffered Saline with Tween 20: powder for 1 L of 10X (sc-362313)
Tris Buffered Saline with Tween 20: powder for 5 L of 10X (sc-362314)
Also available without Tween 20:
Tris Buffered Saline: 1 L of 1X (sc-362185)
Tris Buffered Saline: 1 L of 10X (sc-24951)
Tris Buffered Saline: 100 ml of 10X (sc-362303)
Tris Buffered Saline: 500 ml of 10X (sc-362304)
Tris Buffered Saline: 1 L of 20X (sc-362186)
Tris Buffered Saline: 5 L of 20X (sc-362305)
Tris Buffered Saline: powder for 100 ml of 10X (sc-362188)
Tris Buffered Saline: powder for 500 ml of 10X (sc-362306)
Tris Buffered Saline: powder for 1 L of 10X (sc-362307)
Tris Buffered Saline: powder for 5 L of 10X (sc-362308)
2. Doran, TM, et al. Bioorg Med Chem Lett. 2016. 25(21): 4910-4917. PMID: 26067174
3. Kemper, C. et al. Bio Protoc. 2014. 4(16):e1205. PMID: 29094056
Additional sizes and formats available:
Tris Buffered Saline with Tween 20: 1 L of 1X (sc-362187)
Tris Buffered Saline with Tween 20: 100 ml of 10X (sc-362309)
Tris Buffered Saline with Tween 20: 500 ml of 10X (sc-362310)
Tris Buffered Saline with Tween 20: 1 L of 10X (sc-24953)
Tris Buffered Saline with Tween 20: 1 L of 20X (sc-281695)
Tris Buffered Saline with Tween 20: 5 L of 20X (sc-362311)
Tris Buffered Saline with Tween 20: powder for 100 ml of 10X (sc-362190)
Tris Buffered Saline with Tween 20: powder for 500 ml of 10X (sc-362312)
Tris Buffered Saline with Tween 20: powder for 1 L of 10X (sc-362313)
Tris Buffered Saline with Tween 20: powder for 5 L of 10X (sc-362314)
Also available without Tween 20:
Tris Buffered Saline: 1 L of 1X (sc-362185)
Tris Buffered Saline: 1 L of 10X (sc-24951)
Tris Buffered Saline: 100 ml of 10X (sc-362303)
Tris Buffered Saline: 500 ml of 10X (sc-362304)
Tris Buffered Saline: 1 L of 20X (sc-362186)
Tris Buffered Saline: 5 L of 20X (sc-362305)
Tris Buffered Saline: powder for 100 ml of 10X (sc-362188)
Tris Buffered Saline: powder for 500 ml of 10X (sc-362306)
Tris Buffered Saline: powder for 1 L of 10X (sc-362307)
Tris Buffered Saline: powder for 5 L of 10X (sc-362308)
References:
1. Thomson, BR, et al. Methods Mol Biol. 2019. 1846: 153-160. PMID: 302427582. Doran, TM, et al. Bioorg Med Chem Lett. 2016. 25(21): 4910-4917. PMID: 26067174
3. Kemper, C. et al. Bio Protoc. 2014. 4(16):e1205. PMID: 29094056
Utilisation :
Dilute to a 1X solution with ddH20. Filter solution through a 0.22 μm filter into a sterile flask. Makes 20 L of 1X TBST buffer.
Store the buffer solution at +4°C. Final concentration of Tween-20 (1X solution) = 0.05%.
État Physique :
Liquid
pH :
When diluted to a 1X concentration, the pH will be ~7.4.
STOCKAGE :
Store at 4° C
Pour la Recherche Uniquement. Non conforme pour le Diagnostic ou pour une Utilisation Thérapeutique.
Télécharger la Fiche de Sécurité/ SDS (MSDS)
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SAMPLE Certificate of Analysis (COA)
Tris Buffered Saline with Tween 20: 1 L of 20X SAMPLE Certificate of AnalysisCERTIFICAT D'ANALYSE
Numéro de catalogue
Numéro de Lot
Envoyer
SAMPLE Certificate of Analysis (COA)
Catalog Number:sc-281695
Lot Number:SAMPLE
Product Name:Tris Buffered Saline with Tween 20: 1 L of 20X
| Test | Specification | Results |
|---|---|---|
| Appearance | Slightly yellow liquid, foams when shaken | |
| pH | 7.276 | |
| Sterility | Filtered with 0.2 µm filter |
TEST CONDITIONS
Exp. Date: 9/26/2027