Ubiquitin Antibody (P4D1) is an IgG₁ κ mouse monoclonal Ubiquitin antibody (also designated Ub antibody) that detects the Ubiquitin protein of mouse, rat, human and Drosophila melanogaster origin by WB, IP, IF, IHC(P), FCM and ELISA. Ubiquitin Antibody (P4D1) is available as both the non-conjugated anti-Ubiquitin antibody form, as well as multiple conjugated forms of anti-Ubiquitin antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor^® conjugates. Ubiquitin (Ub) is among the most phylogenetically conserved proteins known. The primary function of Ubiquitin is to clear abnormal, foreign and improperly folded proteins by targeting them for degradation by the 26S Proteosome. This small, 76 amino acid protein can be covalently attached to cellular proteins via an isopeptide linkage between the carboxy terminal group of Ubiquitin and lysine amino groups on the acceptor protein. For proteolysis to occur, Ubiquitin oligomers must be assembled. Ubiquitin chains on proteolytic substrates are commonly found to have an isopeptide bridge between Lys 48 of one Ubiquitin molecule and the carboxy-terminus of a neighboring Ubiquitin molecule. Ubiquitin also plays a role in regulating signal transduction cascades through the elimination inhibitory proteins, such as IκB-α and p27.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

References:

1. Ubiquitin, proteasomes, and the regulation of intracellular protein degradation.  |  Hochstrasser, M. 1995. Curr Opin Cell Biol. 7: 215-23. PMID: 7612274
2. Role of the ubiquitin-proteasome pathway in regulating abundance of the cyclin-dependent kinase inhibitor p27.  |  Pagano, M., et al. 1995. Science. 269: 682-5. PMID: 7624798
3. Ubiquitin and the enigma of intracellular protein degradation.  |  Jennissen, HP. 1995. Eur J Biochem. 231: 1-30. PMID: 7628459
4. Ubiquitin in homeostasis, development and disease.  |  Muller, S. and Schwartz, LM. 1995. Bioessays. 17: 677-84. PMID: 7661849
5. The ubiquitin-proteasome proteolytic pathway.  |  Ciechanover, A. 1994. Cell. 79: 13-21. PMID: 7923371
6. The ubiquitin-mediated proteolytic pathway: mechanisms of recognition of the proteolytic substrate and involvement in the degradation of native cellular proteins.  |  Ciechanover, A. and Schwartz, AL. 1994. FASEB J. 8: 182-91. PMID: 8119489
7. Protein degradation or regulation: Ub the judge.  |  Hochstrasser, M. 1996. Cell. 84: 813-5. PMID: 8601303
8. Site-specific phosphorylation of IkappaBalpha by a novel ubiquitination-dependent protein kinase activity.  |  Chen, ZJ., et al. 1996. Cell. 84: 853-62. PMID: 8601309

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Ubiquitin Antibody (P4D1) has 3,237 citations in a variety of scientific publications.

Primary antibodies like Anti-Ubiquitin Antibody (P4D1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.