ASM10521-P594-100 | ULK2 Antibody Hexabiogen

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ULK2 Polyclonal Antibody (ASM10521). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ULK2 Polyclonal Antibody (ASM10521) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasmic Vesicle Membrane, Peripheral Membrane Protein. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ULK2 Antibody (D) Composite.
Western blot analysis of Human HeLa cell lysates showing detection of 112.7 kDa ULK2 protein using Rabbit Anti-ULK2 Polyclonal Antibody (ASM10521). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human HeLa cell lysates. Load: 15 µg . Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-ULK2 Polyclonal Antibody (ASM10521) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 112.7 kDa. Other Band(s): ~70 kDa.

Product Information
Application Applications Legend: WB=Western Blot IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin-embedded Sections) IHC-F=Immunohistochemistry (Frozen Sections) IF=Immunofluorescence FC=Flow Cytopmetry IC=Immunochemistry ICC=Immunocytochemistry E=ELISA IP=Immunoprecipitation DB=Dot Blot CHIP=Chromatin Immunoprecipitation FA=Fluorescence Assay IEM=Immunoelectronmicroscopy EIA=Enzyme Immunoassay	WB, ICC/IF
Primary Accession	Q8IYT8
Other Accession	NP_001136082.1
Host	Rabbit
Reactivity	Human, Rat
Clonality	Polyclonal
Description	Rabbit Anti-Human ULK2 Polyclonal
Target/Specificity	Detects ~100 kDa.
Other Names	Serine/threonine-protein kinase ULK2 Antibody, EC:2.7.11.1 Antibody, Unc 51 (C. elegans) like kinase 2 Antibody, ULK2_HUMAN Antibody, KIAA0623 Antibody, ATG1B Antibody, Serine/threonine protein kinase ULK2 Antibody, Unc-51-like kinase 2 Antibody, Unc 51 like autophagy activating kinase 2 Antibody, Unc51.2 Antibody, ULK2 Antibody, Unc 51 like kinase 2 Antibody,
Immunogen	Synthetic peptide from the mid-protein of Human ULK2 (aa. 558-569)
Purification	Peptide Affinity Purified
Storage	-20ºC
Storage Buffer	PBS, 50% glycerol, 0.09% sodium azide
Shipping Temperature	Blue Ice or 4ºC
Certificate of Analysis	A 1:1000 dilution of SPC-641 was sufficient for detection of ULK2 in 15 µg of Human HeLa Cell Lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization	Cytoplasmic Vesicle Membrane \| Peripheral Membrane Protein

ULK2 Antibody