Aspartate BioAssay™ Kit

Aspartate, a nonessential amino acid, is a precursor to several other amino acids and is an excitatory neurotransmitter. Aspartate is involved in the urea cycle and gluconeogenesis. The Aspartate BioAssay™ Kit provides a simple, direct and automation-ready procedure for measuring aspartate concentration. Aspartate is converted into pyruvate which is then oxidized with the conversion of the dye into a colored and fluorescent form. The color intensity of the oxidized dye at 570nm or fluorescence intensity at lex/em =530/585nm is directly proportional to the aspartate concentration in the sample.

For quantitative determination of aspartate and evaluation of drug effects on aspartate metabolism.

Direct Assays: aspartate in plasma, serum, tissue and culture media.
Drug Discovery/Pharmacology: effects of drugs on aspartate metabolism.

2-400um aspartate for colorimetric assays and 1-50um for fluorometric assays.

OD, FL: 2, 1um

Developer, 1x1ml
Dye Reagent, 1x120ul
ODC Enzyme, 1x120ul
AST Enzyme, 1x240ul
Cosubstrate, 1x600ul
Aspartate Standard, 1x400ul

Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.