Dolichos biflorus Lectin (DBA) - Pure

Référence 21510042-2

Conditionnement : 10mg

Marque : BioWorld

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Téléphone : +1 850 650 7790


Dolichos biflorus agglutinin (DBA) is isolated from horse gram and purified by affinity chromatography. DBA is a glycoprotein with a molecular weight of about 111,000 and consists of 4 subunits of equal size. This lectin has an isoelectric point between pH 4.6 and pH 5.5. It has a carbohydrate specificity toward ?-N-acetylgalactosamine and elutes with the sugar GalNAc.
DBA has been used as a general marker of developing renal collecting ducts, which can then be used to establish secretion status in blood group A individuals by hemagglutination inhibition and for detecting blood typing. DBA is over thousand times more active in agglutinating blood group A1 cells versus A2 cells, and is used to distinguish these blood types in routine serology applications, and has potential applications in examining tumors which affect the levels of blood group substances.
Reconstitute with sterile buffer. Calcium, manganese, magnesium, and zinc ions are required for binding.


Glycobiology, Blotting, Immunohistochemistry


Dvořánková, B., Motlík, J., Holíková, Z., Vacík, J., & Smetana, K. (2012). Dolichos biflorus agglutinin-binding site expression in basal keratinocytes is associated with cell differentiation. Biology of the Cell, 94(6), “373.

Liu, T., Liu, R., Zhang, S., Guo, K., Zhang, Q., Li, W., & Liu, Y. (2017). Sorafenib induced alteration of protein glycosylation in hepatocellular carcinoma cells. Oncology letters, 14(1), “524.

Valdivia, M., Castañedaâ 9). Spermatogonial stem cells identified by molecular expression of PLZF, integrin β1 and reactivity to Dolichos biflorus agglutinin in alpaca adult testes. Andrologia, 51(6).

Valdivia, M., Reyes, J., Bravo, Z., Cancho, C., Castañeda, S., Limaymanta, O., Woll, P., Santiani, A., & Gonzales, G. F. (2019). In vitro culture of spermatogonial stem cells isolated from adult alpaca (Vicugna pacos) testes analysed withDolichos biflorusby flow cytometry. Andrologia, 51(6).

Zhong, T., Xie, X., Zong, T., Yu, X., Ling, Y., & Kuang, H. (2018). Lectin histochemical analysis of uterine natural killer cells in normal, hydatidiform molar and invasive molar pregnancy. Oncology letters, 16(5), “6464.

Zhou, M. L., Frost, M. R., Xu, Y. C., & Nahm, M. H. (2020). Phosphorylcholine esterase is critical for Dolichos biflorus and Helix pomatia agglutinin binding to pneumococcal teichoic acid. Journal of basic microbiology, 60(10), “915.


Shelf life

2 years

Storage Temperature




Hazmat Ship



Affinity purified

Appearance form

Lyophilized Powder

Molecular Weight

111 kDa

Solubility (@ RT)

Soluble in 0.01M Phosphate Buffered Saline, p

Abbreviation (Lectins Only):



Horse gram

Blood Group Specificity

A1 >> A2

Carbohydrate Specificity




Inhibitory Carbohydrate


Divalent Ions

Ca++, Mn++, Mg++, Zn++

Mitogenic Activity


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