Dolichos biflorus agglutinin (DBA) is isolated from horse gram and purified by affinity chromatography. DBA is a glycoprotein with a molecular weight of about 111,000 and consists of 4 subunits of equal size. This lectin has an isoelectric point between pH 4.6 and pH 5.5. It has a carbohydrate specificity toward ?-N-acetylgalactosamine and elutes with the sugar GalNAc.
DBA has been used as a general marker of developing renal collecting ducts, which can then be used to establish secretion status in blood group A individuals by hemagglutination inhibition and for detecting blood typing. DBA is over thousand times more active in agglutinating blood group A1 cells versus A2 cells, and is used to distinguish these blood types in routine serology applications, and has potential applications in examining tumors which affect the levels of blood group substances.
Reconstitute with sterile buffer. Calcium, manganese, magnesium, and zinc ions are required for binding.

Application

Glycobiology, Blotting, Immunohistochemistry

References

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Liu, T., Liu, R., Zhang, S., Guo, K., Zhang, Q., Li, W., & Liu, Y. (2017). Sorafenib induced alteration of protein glycosylation in hepatocellular carcinoma cells. Oncology letters, 14(1), “524. https://doi.org/10.3892/ol.2017.6177

Valdivia, M., Castañedaâ 9). Spermatogonial stem cells identified by molecular expression of PLZF, integrin β1 and reactivity to Dolichos biflorus agglutinin in alpaca adult testes. Andrologia, 51(6). https://doi.org/10.1111/and.13283

Valdivia, M., Reyes, J., Bravo, Z., Cancho, C., Castañeda, S., Limaymanta, O., Woll, P., Santiani, A., & Gonzales, G. F. (2019). In vitro culture of spermatogonial stem cells isolated from adult alpaca (Vicugna pacos) testes analysed withDolichos biflorusby flow cytometry. Andrologia, 51(6). https://doi.org/10.1111/and.13269

Zhong, T., Xie, X., Zong, T., Yu, X., Ling, Y., & Kuang, H. (2018). Lectin histochemical analysis of uterine natural killer cells in normal, hydatidiform molar and invasive molar pregnancy. Oncology letters, 16(5), “6464. https://doi.org/10.3892/ol.2018.9465

Zhou, M. L., Frost, M. R., Xu, Y. C., & Nahm, M. H. (2020). Phosphorylcholine esterase is critical for Dolichos biflorus and Helix pomatia agglutinin binding to pneumococcal teichoic acid. Journal of basic microbiology, 60(10), “915. https://doi.org/10.1002/jobm.202000177