Maackia amurensis Lectin I (MAL I) (FITC)

Référence M1061-10F-2mg

Conditionnement : 2mg

Marque : US Biological

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Conjugate
FITC

Grade
Molecular Biology Grade

Shipping Temp
Blue Ice

Storage Temp
-20°C

Amur Maackia
This lectin (MAL) is the leucoagglutinin or mitogenic isolectin form from Maackia seeds. This multimeric lectin dissociates into 38,000 and 40,000 molecular weight bands in SDS gels in the presence of reducing agents and is a single 75,000D band without reducing agents. It has an isoelectric point about pH 4.6 and has been reported to have a molecular weight of about 130,000 by gel filtration. This lectin binds glycoconjugates having galactosyl (b-1,4) N-acetylglucosamine structures. Maackia amurensis lectin I seems to tolerate substitution of N-acetyllactosamine with sialic acid at the 3 position of galactose. However, MAL I does not appear to bind this structure when substitution with sialic acid is on the 6 position of galactose.
Fluorescein labeled Maackia Amurensis Lectin is produced by using the highest quality fluorescein isothiocyanate, our affinity-purified lectin, and special conjugation procedures. Fluorescein labeled Maackia Amurensis Lectin has an appropriate number of fluorochromes bound which provide the maximum fluorescence and optimum staining characteristics for this particular lectin. This lectin is supplied essentially free of unconjugated fluorochromes and inactive lectin.

Applications:
Suitable for use in Immunofluorescence. Other applications not tested.

Recommended Dilutions:
Immunofluorescence: 5-20ug/ml.
Optimal dilutions to be determined by the researcher.

Inhibiting Sugar:
200mM lactose

Sugar Specificity:
Galatose, lactose

Excitation:
495-500nm

Emission:
514-521nm

F/P (molar):
10.2

Active Conjugate:
As Reported

Storage and Stability:
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 6 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Source
Maackia amurensis seeds

Concentration
~2mg/ml

Form
Supplied as a liquid in 10mM HEPES, 0.15M sodium chloride, pH 7.5, 0.08% sodium azide. 0.1mM Ca++. Labeled with Fluorescein isothiocyanate (FITC).


Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.