MHC tetramers are complexes of 4 MHC molecules (Major Histocompatibility Complex) wich are associated with a specific peptide and bound to a fluorochrome.
Class I tetramers bind to a distinct set of T cell receptors (TRCs) on a subset of T CD8+ cells, Class II tetramers bind to a distinct population of T CD4+ cells.
The patented MHC iTag tetramers reduce non-specific bindings to allow unique detection and enumeration of Ag specific T cells.
Thus, by mixing Tetramers with PBMCs or
whole blood and using flow cytometry as a detection system, a count of
all CD4+ or CD8+ T cells that are specific for one peptide and its
matched MHC allele is provided, regardless of functionality.
- The only method to detect antigen specific CD4+ or CD8+ T cells ex vivo
- Immune responses are targeted to specific allele
- Tetramer staining is quantitative
- Does not require the use of radioactive isotopes
- Rapid: allowing fresh blood (or tissue-derived) sample to be analyzed
- Large number of samples can be processed using flow cytometer
- Tetramer-labeled cells remain viable
- All specific CTLs are detected
- Tetramers can be customized for unique applications
Specific Mutation reducing non specific binding:
The class 1 MHC present a proprietary mutation of HLA alpha 3 domain region which greatly reduces the non specific binding
- Infectious diseases: VIH-SIDA, EBV, CMV, HPV, HBV, HCV, Influenza, Measles, Malaria, TB and RSV
- Cancers: Breast, Prostate, Melanoma, Colon, Lung, Cervical, Ovairian et Leukemia
- Autoimmunes diseases: Diabetes, Lyme disease, Multiple sclerosis, Rheumatoid arthritis, Autoimmun vitiligo
- Transplantation: EBV and CMV
Developed to your:
- fluorochrome (PE or APC)