CD3 antibody [17A2] (APC)
Référence GTX01458-07-100ug
Conditionnement : 100ug
Marque : Genetex
Contactez votre distributeur local :
Téléphone : +1 850 650 7790
![Anti-CD3 antibody [17A2] (APC) used in Flow cytometry (FACS). GTX01458-07](http://www.genetex.com/templates/default/js/lightbox/images/loading.gif "Anti-CD3 antibody [17A2] (APC) used in Flow cytometry (FACS). GTX01458-07")
Host | Rat |
|---|---|
Clonality | Monoclonal |
Clone Name | 17A2 |
Isotype | IgG2b |
Application | FACS |
Reactivity | Mouse |
| Package | 100 μg |
Datasheet File View product citations for antibody GTX01458-07 on CiteAb
PRODUCT
Summary
The 17A2 antibody reacts with the mouse CD3 complex, comprised of CD3 epsilon, CD3 gamma and CD3 delta. These integral membrane protein chains assemble with additional chains of the T cell receptor (TCR), as well as CD3 zeta chain, to form the T cell receptor – CD3 complex. Together with co-receptors CD4 or CD8, the complex serves to recognize antigens bound to MHC molecules on antigen-presenting cells. Such interactions promote T cell receptor signaling (T cell activation) and can result in a number of cellular responses including proliferation, differentiation, production of cytokines or activation-induced cell death. CD3 is differentially expressed during thymocyte-to-T cell development and on all mature T cells. The 17A2 antibody is a widely used phenotypic marker for mouse T cells. In addition, as the CD3e chain within the TCR complex contains intracellular signaling domains, binding of 17A2 antibody to CD3 can induce cell activation (use format suitable for functional assays).
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application | Recommended Dilution |
|---|---|
| FACS | Assay dependent |
Not tested in other applications.
PROPERTIES
Form
Liquid
Buffer
10mM NaH₂PO₄, 150mM NaCl, 0.1% Gelatin
Preservative
0.09% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Purification
Purified by affinity chromatography
From tissue culture supernatant
From tissue culture supernatant
Conjugation
Allophycocyanin (APC)
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
CD3 antigen, delta polypeptide , CD3 antigen, epsilon polypeptide , CD3 antigen, gamma polypeptide , CD247 antigen , T3d , AI504783 , CD3 , CD3epsilon , T3e , Ctg-3 , Ctg3 , T3g , 4930549J05Rik , A430104F18Rik , AW552088 , Cd3 , Cd3-eta , Cd3-zeta , Cd3h , Cd3z , Cd3zeta , T3z , Tcrk , Tcrz
Cellular Localization
Cell membrane , Membrane; Single-pass type I membrane protein
Database
DATA IMAGES
| | GTX01458-07 FACS Image FACS analysis of mouse C57Bl/6 splenocytes using GTX01458-07 CD3 antibody [17A2] (APC). |
Clone Reference
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Submit a Reference REVIEW
Clone Reference
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1989; Production and characterization of a rat monoclonal antibody against the murine CD3 molecular complex.
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