CD52 Rat Monoclonal Antibody [Clone ID: YTH34.5]

Specifications

Product Data
Clone Name	YTH34.5
Applications	FC
Recommended Dilution	Flow Cytometry (Neat): Use 10 ul of the suggested working dilution to label 10e6 cells or 100 ul whole blood.
Reactivities	Human, Monkey
Host	Rat
Isotype	IgG2b
Clonality	Monoclonal
Immunogen	Human lymphocytes.
Specificity	This antibody reacts with the human CD52 antigen, also known as CAMPATH-1. Humanised versions of CAMPATH-1 specific antibodies are currently in clinical trials for the treatment of a range of lymphoid malignancies.
Formulation	PBS containing 0.09% Sodium Azide as preservative and 1% Bovine Serum Albumin as stabilizer. Label: FITC State: Liquid purified IgG fraction. Label: Fluorescein Isothiocyanate Isomer 1
Concentration	lot specific
Purification	Affinity Chromatography on Protein A.
Conjugation	FITC
Storage	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Stability	Shelf life: one year from despatch.
Gene Name	CD52 molecule
Database Link	Entrez Gene 1043 Human UniProt ID P31358
Background	The CD52 antigen is a remarkably small peptide that is heavily glycosylated, and attached to the cell surface membrane via a GPI link. The apparent molecular mass of the antigen on SDS-PAGE is 25-29kD. CD52 is expressed at high density by lymphocytes, monocytes, eosinophils, thymocytes and macrophages. It is expressed by most lymphoid derived malignancies, although expression on myeloma cells is variable.
Synonyms	HE5, CDw52
Reference Data