Formate dehydrogenase Rabbit Polyclonal Antibody

CAT#: AP21253BT-N

Formate dehydrogenase rabbit polyclonal antibody, Biotin


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Specifications

Product Data
Applications ELISA, ID, IF, IP, R, WB
Recommended Dilution This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and Immunofluorescence or Histochemical techniques), to prepare an insoluble immuno-affinity adsorbent, for labelling with a marker of choice.
Working Dilutions:
Non-precipitating antibody-binding techniques: 1/1,000-1/5,000.
Reactivities Yeast
Host Rabbit
Isotype IgG
Clonality Polyclonal
Immunogen Formate Dehydrogenase is isolated and purified from Yeast.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Specificity The antibody recognizes Formate Dehydrogenase from Yeast.
The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, Single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and enzyme inhibition.
Cross-reactivities against enzymes of other sources may occur but have not been determined.
Formulation PBS, pH 7.2 without preservatives and foreign proteins
Label: Biotin
State: Lyophilized hyperimmune IgG fraction
Molar radio: Biotin/IgG ~6.3
Reconstitution Method Restore by adding 1.0 ml of sterile distilled water
Concentration lot specific
Purification Ammonium Sulphate Precipitation and Ion Exchange Chromatography.
Conjugation Biotin
Storage Store the antibody lyophilized at 2-8°C and reconstituted at 2-8°C for one week or (in aliquots) at -20°C for longer.
If a slight precipitation occurs upon storage, this should be removed by centrifugation.
Stability Shelf life: one year from despatch.
Synonyms FDH1, FDH, FDH3
Reference Data

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