HLA Class II DR Mouse Monoclonal Antibody [Clone ID: 423L]

CAT#: AM05204BT-N

HLA Class II DR mouse monoclonal antibody, clone 423L, Biotin

Conjugation: Unconjugated Biotin FITC


Product Images

Specifications

Product Data
Clone Name 423L
Applications FC
Recommended Dilution Flow Cytometry.
Monitoring of B cells in peripheral blood.
Analysis of B subsets.
Study of B cell activation.
Study of B cell neoplasms.
Reactivities Human, Monkey
Host Mouse
Isotype IgG2a
Clonality Monoclonal
Specificity This antibody recats with human B and T subset Lymphocyte.
Formulation PBS containing 0.2% protein carrier and 0.08% Sodium Azide as preservative.
Label: Biotin
State: Liquid purified IgG fraction.
Concentration lot specific
Conjugation Biotin
Storage Store the antibody undiluted at 2-8°C.
Do Not Freeze!
Stability Shelf life: One year from despatch.
Background Identification of human B cell and T cell subsets associated with approximately 10% of peripheral blood lymphocytes 28-34,000 M.W. surface antigen, also low density on monocytes and macrophages.
Synonyms HLA-DR, HLA class II histocompatibility antigen DR, MHC class II antigen DR
Note Protocol: PBMC:
Add 10 ul of MAB/10e6 PBMC in 100 ul PBS.
Mix gently and incubate for 15 minutes at 2-8°C.
Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze.

WHOLE BLOOD:
Add 10 ul of MAB/100 ul of whole blood.
Mix gently and incubate for 15 minutes at room temperature (20°C).
Lyse the whole blood.
Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze.
See instrument manufacturers instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.
Reference Data

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