Macrophages (Haematopoiesis associated) Mouse Monoclonal Antibody [Clone ID: 25F9]

Specifications

Product Data
Clone Name	25F9
Applications	FC, IHC, WB
Recommended Dilution	Immunohistochemistry on frozen sections. Immunohistochemistry on paraffin sections. Flow cytometry. Western blot. The typical starting working dilution for these applications  is 1:50.
Reactivities	Human, Monkey
Host	Mouse
Isotype	IgG1
Clonality	Monoclonal
Specificity	The monoclonal antibody 25F9 recognises a protein of 86 kD on the cell surface and within the cytoplasm of mature macrophages. The antibody is associated with fully differentiated tissue macrophages both in normal and in diseased tissues, independently of the presence or absence of inflammation. The antigen is absent on freshly isolated monocytes and other blood cells. After 6 to 7 days culture human monocytes become positive. Some melanoma and carcinoma cell lines are also positive. Furthermore the monoclonal antibody 25F9 cross reacts with a subpopulation of macrophages of rhesus monkey, pig alveolar macrophages and Kupffer cells. The monoclonal antibody 25F9 is very useful for macrophage phenotyping.
Formulation	PBS Label: Biotin State: Liquid 0.2 µm filtered Ig fraction Stabilizer: 0.1% bovine serum albumin Preservative: 0.02% sodium azide
Concentration	lot specific
Purification	Protein G
Conjugation	Biotin
Storage	Store at 2 - 8 °C.
Stability	Shelf life: one year from despatch.
Synonyms	Macrophage marker
Reference Data