Mouse FGF9 (Fibroblast Growth Factor 9) CLIA Kit

Storage Instruction:	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time:	3.5h
Detection:	Chemiluminescence

Gene Symbol:	Fgf9
Gene ID:	14180
Uniprot ID:	FGF9_MOUSE
Specificity:	This kit recognizes Mouse FGF9 in samples. No significant cross-reactivity or interference between Mouse FGF9 and analogues was observed.
Sample Type:	Serum, plasma and other biological fluids

Function	Plays an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration. May have a role in glial cell growth and differentiation during development, gliosis during repair and regeneration of brain tissue after damage, differentiation and survival of neuronal cells, and growth stimulation of glial tumors.
Protein Name	Fibroblast Growth Factor 9 Fgf-9 Glia-Activating Factor Gaf Hbgf-9
Database Links	Reactome: R-MMU-109704 Reactome: -MMU-1257604 Reactome: -MMU-190322 Reactome: -MMU-190371 Reactome: -MMU-190372 Reactome: -MMU-190373 Reactome: -MMU-190375 Reactome: -MMU-5654219 Reactome: -MMU-5654221 Reactome: -MMU-5654227 Reactome: -MMU-5654228 Reactome: -MMU-5654687 Reactome: -MMU-5654688 Reactome: -MMU-5654689 Reactome: -MMU-5654693 Reactome: -MMU-5654695 Reactome: -MMU-5654699 Reactome: -MMU-5654700 Reactome: -MMU-5654704 Reactome: -MMU-5654706 Reactome: -MMU-5654710 Reactome: -MMU-5654712 Reactome: -MMU-5654719 Reactome: -MMU-5654720 Reactome: -MMU-5654726 Reactome: -MMU-5654727 Reactome: -MMU-5654732 Reactome: -MMU-5654733 Reactome: -MMU-5673001 Reactome: -MMU-6811558
Cellular Localisation	Secreted
Alternative CLIA Names	Fibroblast Growth Factor 9 CLIA kit Fgf-9 CLIA kit Glia-Activating Factor CLIA kit Gaf CLIA kit Hbgf-9 CLIA kit Fgf9 CLIA kit Fgf-9 CLIA kit
Specificity	This kit recognizes Mouse FGF9 in samples. No significant cross-reactivity or interference between Mouse FGF9 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro CLIA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent A	1 vial, 5 mL	2-8℃ (Protect from light)
Substrate Reagent B	1 vial, 5 mL	2-8℃ (Protect from light)
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	87-98	93
EDTA plasma(n=8)	92-102	97
Cell culture media(n=8)	93-110	101

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	50.82	90.98	407.67	55.79	86.59	407.35
Standard deviation	6.52	9.22	35.30	5.58	8.69	32.30
CV (%)	12.83	10.13	8.66	10.00	10.04	7.93

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance