Mouse IgG IgA IgM (H&L) Antibody Rhodamine Conjugated Pre-Adsorbed

Specifications

Product Data
Applications	FC, IF
Recommended Dilution	IF: 1:1,000 - 1:5,000 FC: 1:500 - 1:2,500 FLISA: 1:10,000 - 1:50,000
Reactivities	Mouse
Host	Goat
Clonality	Polyclonal
Immunogen	Mouse IgG IgA and IgM whole molecule
Specificity	This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum.  No reaction was observed against bovine, horse or human serum proteins.  This reagent is suitable for the detection of all mouse immunoglobulin isotypes and chain combinations.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Method	Restore with deionized water (or equivalent) - Reconstitution Volume: 1.0 mL
Concentration	1.0 mg/mL - lot specific
Conjugation	TRITC
Storage	Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
Note	This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.