Ninhydrin [485-47-2]
Référence HY-D0908-1g
Conditionnement : 1g
Marque : MedChemExpress
Ninhydrin can be used as a chromogenic analytical probe for the quantification of amino acids and proteins.
Nos produits utilisent uniquement pour la recherche. Nous ne vendons pas aux patients.
Ninhydrin Chemical Structure
CAS No. : 485-47-2
This product is a controlled substance and not for sale in your territory.
Based on 1 publication(s) in Google Scholar
Description |
Ninhydrin can be used as a chromogenic analytical probe for the quantification of amino acids and proteins. |
IC50 & Target |
IC50: chromogenic analytical probe[1] |
||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
In Vitro |
Ninhydrin is used in amino acid analysis of proteins. Except proline, Most amino acids can be hydrolyzed and react with ninhydrin. The amino acids are then quantified colorimetrically after separation by chromatography. Ninhydrin reacts with primary and secondary amines producing a blue or purple reaction product: diketohydrindylidene-diketohydrindamine. MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. |
||||||||||||
Masse moléculaire |
178.14 |
||||||||||||
Formule |
C9H6O4 |
||||||||||||
CAS No. |
485-47-2 |
||||||||||||
Appearance |
Solid |
||||||||||||
Color |
Off-white to light yellow |
||||||||||||
SMILES |
O=C1C(O)(O)C(C2=C1C=CC=C2)=O |
||||||||||||
Livraison | Room temperature in continental US; may vary elsewhere. |
||||||||||||
Stockage |
|
||||||||||||
Solvant et solubilité |
In Vitro:
DMSO : 50 mg/mL (280.68 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO) H2O : 25 mg/mL (140.34 mM; ultrasonic and warming and heat to 60°C) Preparing
Stock Solutions
View the Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles. * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol) Concentration (start) × Volume (start) = Concentration (final) × Volume (final) This equation is commonly abbreviated as: C1V1 = C2V2 In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
For the following dissolution methods, please prepare the working solution directly.
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
Dosage mg/kgAnimal weight Dosing volume Number of animals Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration:
mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
|
||||||||||||
Pureté et documentation | |||||||||||||
Références |
|
Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
---|
H2O / DMSO | 1 mM | 5.6136 mL | 28.0678 mL | 56.1356 mL | 140.3391 mL |
5 mM | 1.1227 mL | 5.6136 mL | 11.2271 mL | 28.0678 mL | |
10 mM | 0.5614 mL | 2.8068 mL | 5.6136 mL | 14.0339 mL | |
15 mM | 0.3742 mL | 1.8712 mL | 3.7424 mL | 9.3559 mL | |
20 mM | 0.2807 mL | 1.4034 mL | 2.8068 mL | 7.0170 mL | |
25 mM | 0.2245 mL | 1.1227 mL | 2.2454 mL | 5.6136 mL | |
30 mM | 0.1871 mL | 0.9356 mL | 1.8712 mL | 4.6780 mL | |
40 mM | 0.1403 mL | 0.7017 mL | 1.4034 mL | 3.5085 mL | |
50 mM | 0.1123 mL | 0.5614 mL | 1.1227 mL | 2.8068 mL | |
60 mM | 0.0936 mL | 0.4678 mL | 0.9356 mL | 2.3390 mL | |
80 mM | 0.0702 mL | 0.3508 mL | 0.7017 mL | 1.7542 mL | |
100 mM | 0.0561 mL | 0.2807 mL | 0.5614 mL | 1.4034 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Ninhydrin Related Classifications
-
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.
Keywords:
Ninhydrin485-47-2Fluorescent DyeDyechromogenicprobeaminoproteinInhibitorinhibitorinhibit