Rat IgE (Fc specific) Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, ID, IF, IHC, IP
Recommended Dilution	This antibody can be used In Immunocytochemical and Immunohistochemical staining for the detection of IgE at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates. To identify and measure IgE in Rat serum or other body fluids. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Dilutions: 1/20-1/80.
Reactivities	Rat
Host	Goat
Isotype	IgG
Clonality	Polyclonal
Immunogen	Purified homogenous IgE isolated from Rat serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Specificity	The reactivity of the antiserum is directed to the Fc subunit of the IgE molecule, which expresses strict (class) specificity. In Immunoelectrophoresis and Radial in immunodiffusion using various antiserum concentrations against rat serum, a single precipitin line has been obtained which shows a reaction of identity with the precipitin lines obtained with the purified IgE used as immunogens. It does not react with IgG including all subclasses, IgG/Fab fragments, IgM and IgA or any non-Ig protein in rat serum, as tested by immunoelectrophoresis and Double Radial Immunodiffusion. The antiserum also react with membrane-bound IgE in peripheral blood cells as tested by Immunofluorescence microscopy. Cross-reactivity: This immunoconjugate is not species-specific since inter-species cross-reactivity is a normal feature of antisera to immunoglobulins. Cross-reactivity of this conjugate has not been tested in detail.
Formulation	PBS, pH 7.2 without preservatives. Label: FITC State: Lyophilized IgG fraction. Label: Fluorescein Isothiocyanate Absorption emission: 492 nm / 515 nm Molar radio: Fluorochrome/IgG ~2.1
Reconstitution Method	Restore by adding 1 ml of sterile distilled water.
Concentration	10.0 mg/ml
Purification	Hyperimmune antisera with strong precipitating activity are selected for fractionation by saltprecipitation and purification of the IgG fraction by DEAE-chromatography.
Conjugation	FITC
Storage	Store lyophilized at 2-8°C and reconstituted at 2-8°C for one week or (in aliquots) at -20°C for longer. Avoid Repeated thawing and freezing.
Note	Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies cross-reacting with other with other plasma proteins. Special attention is given to the elimination of antibodies to the common Fab portion of immunoglobulins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.