Rat IgM (Mu chain specific) Goat Polyclonal Antibody

Specifications

Product Data
Recommended Dilution	Suitable for immunomicroscopy and flow cytometry as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. Recommended dilutions: The maximum amount of reagent required to stain 1 x 10e6 cells in flow cytometry is approximately 1.0 µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining. As a general guideline dilutions of 1:50 to 1:200 should be suitable for most applications.
Reactivities	Rat
Host	Goat
Clonality	Polyclonal
Immunogen	Rat IgM mu heavy chain.
Specificity	This product was prepared from monospecific antiserum by Immunoaffinity chromatography using Rat IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by Immunoelectro-phoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Goat Serum, Rat IgM, and Rat Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rat IgG F(c) or human, bovine or horse serum proteins.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, with 0.01% (w/v) Sodium Azide as preservative and 10 mg/ml BSA (IgG and Protease free) as stabilizer Label: PE State: Lyophilized F(ab')2 fragments Label: R-Phycoerythrin (, MW 240,000 daltons) isolated from seaweed. Phycobiliprotein concentration: 0.5 mg/ml (determined by absorbance = 82.0 at 565 nm for a 1% solution for only those molecules to which at least one molecule of active antibody is bound) Absorption emission: 490 nm, 545 nm and 565 nm / 580 nm
Reconstitution Method	Restore with 1.0 ml of deionized water (or equivalent).
Purification	Immunoaffinity chromatography.
Conjugation	PE
Storage	Store vial at 2-8°C prior to restoration. Restore with deionized water (or equivalent); centrifuge product if not completely clear after standing at room temperature. This product is stable at 2-8°C as an undiluted liquid. DO NOT FREEZE. Protect from light. Dilute only prior to immediate use.