Streptococcus Group A Goat Polyclonal Antibody

Specifications

Product Data
Applications	IF
Recommended Dilution	ELISA (functions both as capture and detection antibody). Immunofluorescence assay: a starting range of 1:10-1:50 is suggested. Direct Fluorescence assay staining of target antigens in a permissive tissue culture system. Acetone fixation of the antigen source is recommended prior to staining. Pre-block slide with 10 % normal goat serum.
Reactivities	Streptococcus sp.
Host	Goat
Clonality	Polyclonal
Immunogen	Streptococci, Group A
Specificity	The antibody recognizes a type specific carbohydrate for group A Streptococcus. Does not cross-react with other Strep groups.
Formulation	0.01 M PBS, pH 7.2, containing 0.09% sodium azide as preservative and 10 mg/ml BSA as stabilizer Label: FITC State: Liquid purified Ig fraction Label: High purity Isomer I of fluorescein isothiocyanate Care is taken to ensure complete removal of any free fluorescein from the final product Molar radio: F:P ratio = 5.4
Concentration	lot specific
Purification	Immunoaffinity chromatography
Conjugation	FITC
Storage	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.
Stability	Shelf life: one year from despatch.
Background	The genus Streptococcus is comprised of a wide variety of both pathogenic and commensal gram positive bacteria which are found to inhabit a wide range of hosts, including humans, horses, pigs and cows. Within the host, streptococci are often found to colonize the mucosal surfaces of the mouth, nose and pharynx. Streptococci can be divided into many groups on the basis of antigenic differences in group-specific polysaccharides located in the bacterial cell wall. More than 20 serologic groups have been identified and designated by letters, eg, A, B, C. Group A streptococci, cause a wide-range of disease in humans, from mild sore throats to life-threatening invasive disease such as necrotising fasciitis. Streptococcus pyogenes (a group A Streptococcus) is one of the most important pathogens encountered in clinical practice. Group A streptococci (GAS) are gram-positive, nonmotile, non-spore-forming organisms that appear as pairs or short-to-moderate-sized chains. Group A organisms can be identified by enzyme immunoassays.
Note	There is potential non-specific reactivity with each organisms as group C Streptococcus and Staphyloccus due to the presence of protein A like proteins (Staphyloccus) and protein C like proteins (Streptococcus C) present on the intact surface of these bacteria. These proteins bind any immunoglobulin (including the FITC Conjugate) and therefore will appear to fluoresce (non-specifically).
Reference Data