Tom20 (F-10)
Référence sc-17764
Conditionnement : 200µg/ml
Marque : Santa Cruz Biotechnology
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Anticorps Tom20 (F-10): sc-17764
- L'Anticorps Tom20 (F-10) est un monoclonal de souris IgG2a λ L'Anticorps Tom20, cité dans 615 publications, fourni en 200 µg/ml
- raised against amino acids 1-145 of Tom20 human origin
- L'Anticorps Tom20 (F-10) est recommandé pour la détection de Tom20 d'origine mouse, rat et human par WB, IP, IF, IHC(P) et ELISA
- Anti-L'Anticorps Tom20 (F-10) est disponible conjugué à l'agarose pour IP; à l'HRP pour WB, IHC(P) et ELISA; et soit à la phycoerythrin ou FITC pour IF, IHC(P) et FCM
- aussi disponible conjugué à l'Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 ou Alexa Fluor® 647 pour WB (RGB), IF, IHC(P) et FCM
- aussi disponible conjugué à l'Alexa Fluor® 680 ou Alexa Fluor® 790 pour WB (NIR), IF et FCM
- 2a BP-HRP">m-IgG2a BP-HRP et m-IgGλ BP-HRP sont les réactifs secondaires recommandés pour la détection de l'anticorps Tom20 (F-10) pour des applications de WB and IHC(P). Ces réactifs sont maintenant proposés en kit avec l'anticorps Tom20 (F-10) ().
ACCÈS RAPIDE AUX LIENS
, Tom1L-1, Tom20, Tom22, Tom34, Tom40, Tom6 et Tom70
Tom20 Antibody (F-10) is an IgG2a λ mouse monoclonal Tom20 antibody (also designated TOMM20 antibody) that detects the Tom20 protein of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. Tom20 Antibody (F-10) is available as both the non-conjugated anti-Tom20 antibody form, as well as multiple conjugated forms of anti-Tom20 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Tom20 is a member of the multisubunit complex Tom (mitochondrial preprotein translocases of the outer membrane). Tom facilitates the import of nucleus-encoded precursor proteins across the mitochondrial outer membrane. The Tom machinery consists of import receptors for the initial binding of cytosolically synthesized preproteins and a general import pore (GIP) for the membrane translocation of various preproteins into the mitochondria. The import receptors include Tom20 and Tom22, which form a heteromeric receptor complex that initiates the insertion of newly synthesized proteins into the outer membrane and then directs the precursor protein into the GIP. Tom20 Antibody (F-10) is specific for mammalian Tom20 protein. In yeast, Tom22 is the essential component of the import receptor complex as it functions as both a receptor for the preproteins and serves as a docking point for both Tom20 and the GIP. Tom22 directly associates with Tom40, the major component of the GIP, and thereby forms a stable interaction between the two core complexes to facilitate the fluid movement of preproteins into the mitochondria. The insertion of Tom40 into the Tom machinery requires the initial binding of Tom40 to Tom20 and leads to the efficient incorporation of Tom40 precursors into preexisting Tom complexes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
References:
- Distribution of binding sequences for the mitochondrial import receptors Tom20, Tom22, and Tom70 in a presequence-carrying preprotein and a non-cleavable preprotein. | Brix, J., et al. 1999. J Biol Chem. 274: 16522-30. PMID: 10347216
- Biogenesis of Tom40, core component of the TOM complex of mitochondria. | Rapaport, D. and Neupert, W. 1999. J Cell Biol. 146: 321-31. PMID: 10427088
- Tom22 is a multifunctional organizer of the mitochondrial preprotein translocase. | van Wilpe, S., et al. 1999. Nature. 401: 485-9. PMID: 10519552
- The TOM core complex: the general protein import pore of the outer membrane of mitochondria. | Ahting, U., et al. 1999. J Cell Biol. 147: 959-68. PMID: 10579717
- The transport machinery for the import of preproteins across the outer mitochondrial membrane. | Ryan, MT., et al. 2000. Int J Biochem Cell Biol. 32: 13-21. PMID: 10661891
- Mitochondrial protein import. Tom40 plays a major role in targeting and translocation of preproteins by forming a specific binding site for the presequence. | Rapaport, D., et al. 1997. J Biol Chem. 272: 18725-31. PMID: 9228044
- Functional analysis of human mitochondrial receptor Tom20 for protein import into mitochondria. | Yano, M., et al. 1998. J Biol Chem. 273: 26844-51. PMID: 9756929
- Preprotein translocase of the outer mitochondrial membrane: molecular dissection and assembly of the general import pore complex. | Dekker, PJ., et al. 1998. Mol Cell Biol. 18: 6515-24. PMID: 9774667
Espèces | Nom du Gène | Gene ID | Localisation chromosomique | Numéro d'accession de l'isoforme (ARNm) | Numéro d'accès aux protéines | Numéro OMIM™ |
---|---|---|---|---|---|---|
Human | TOMM20 | Entrez Gene ID: 9804 | 1q42.3 | NM_014765 | UniProt ID: Q15388 | 601848 |
Mouse | Tomm20 | Entrez Gene ID: 67952 | 8 E2 | NM_024214 | UniProt ID: Q9DCC8 | N/A |
Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz® conjugates which eliminates the need for a secondary antibody.
Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Tom20 Antibody (F-10) has 615 citations in a variety of scientific publications.
Primary antibodies like Anti-Tom20 Antibody (F-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.
L'anticorps Tom20 préféré est l'Anticorps Tom20 (29): sc-136211.
Hello! Can I use this antibody for Flow Cytometry? If so, with what dilution?
What is the epitope sequence for this antibody? Is it the whole Tom20 protein sequence?
What fixative is recommended for this antibody? Can I use 4% PFA? Trichloroacetic Acid (TCA)?
Can I use Tom20 抗体 (F-10): sc-17764 in pig ?
Do you have an antibody like sc-11415 against TOMM20 & produced in rabbit?
hi, Sir or Madam, your website showed Tom20 antibody in your company also reacted with the drosophila in WB, can you send me the article that has ever used this antibody in flies? thanks a lot.
this antibody should replace the old polyclonal "sc-11415" antibody. However, this one is mouse, and i am looking for an antibody coming from rabbit. Is it possible to still order the old antibody?
What is the minimum amount of protein required for signal? I am currently loading 5 ug of protein as this is ideal for the other Abs I am working with. I have tried to dilute the TOM20 F-10 antibody at 1:500 and 1:100 without a hint of a band.
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