Uricase antibody (Peroxidase)
Référence orb344232-25ul
Conditionnement : 25ul
Marque : Biorbyt
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Uricase antibody (Peroxidase)
Catalog Number: orb344232
| Catalog Number | orb344232 |
|---|---|
| Category | Antibodies |
| Description | Uricase antibody (Peroxidase) |
| Species/Host | Goat |
| Clonality | Polyclonal |
| Tested applications | ELISA, IP, WB |
| Reactivity | Bacteria |
| Isotype | IgG |
| Immunogen | Anti-uricase Antibody was produced by repeated immunizations with bacillus uricase protein. |
| Concentration | 1.0 mg/mL |
| Dilution range | ELISA: 1:1,000 - 1:2,500, IP: 1:100, WB: 1:500 - 1:1,000 |
| Form/Appearance | Liquid (sterile filtered) |
| Purity | Anti-Uricase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Uricase [Bacillus species]. Cross reactivity against Uricase from other sources is unknown. |
| Conjugation | HRP |
| UniProt ID | Q5WBJ3 |
| NCBI | YP_177228.1 |
| Storage | Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing. |
| Buffer/Preservatives | 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! |
| Alternative names | goat anti-uricase antibody, HRP conjugated goat an |
| Note | For research use only |
| Application notes | Anti-Uricase antibody has been tested by ELISA and western blot and is suitable for IP. Researchers should determine optimal titers for applications that are not stated below. |
| Expiration Date | 12 months from date of receipt. |
Western blot analysis of purified Uricase under reducing conditions (Lane1), purified Uricase under reducing conditions (Lane2), purified Uricase under non-reducing conditions (Lane3), purified Uricase under non-reducing (Lane3) conditions Load: ~1.0 ug (