IPTG [367-93-1]

Référence HY-15921-10g

Conditionnement : 10g

Marque : MedChemExpress

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IPTG is a molecular mimic of allolactose, a lactose metabolite that triggers transcription of the lac operon, and it is therefore used to induce protein expression where the gene is under the control of the lac operator.

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IPTG Chemical Structure

CAS No. : 367-93-1

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    Screening of thymidylate synthase (TYMS) overexpression conditions.
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    IPTG is a molecular mimic of allolactose, a lactose metabolite that triggers transcription of the lac operon, and it is therefore used to induce protein expression where the gene is under the control of the lac operator.

    In Vitro

    IPTG uptake by E. coli can be independent of the action of lactose permease, since other transport pathways are also involved. At low concentration, IPTG enters cells through lactose permease, but at high concentrations (typically used for protein induction), IPTG can enter the cells independently of lactose permease.

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    • [1]. Isopropyl β-D-1-thiogalactopyranoside

    • [1]. Isopropyl β-D-1-thiogalactopyranoside

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO / H2O 1 mM 4.1964 mL 20.9820 mL 41.9639 mL 104.9098 mL
    5 mM 0.8393 mL 4.1964 mL 8.3928 mL 20.9820 mL
    10 mM 0.4196 mL 2.0982 mL 4.1964 mL 10.4910 mL
    15 mM 0.2798 mL 1.3988 mL 2.7976 mL 6.9940 mL
    20 mM 0.2098 mL 1.0491 mL 2.0982 mL 5.2455 mL
    25 mM 0.1679 mL 0.8393 mL 1.6786 mL 4.1964 mL
    30 mM 0.1399 mL 0.6994 mL 1.3988 mL 3.4970 mL
    40 mM 0.1049 mL 0.5245 mL 1.0491 mL 2.6227 mL
    50 mM 0.0839 mL 0.4196 mL 0.8393 mL 2.0982 mL
    60 mM 0.0699 mL 0.3497 mL 0.6994 mL 1.7485 mL
    80 mM 0.0525 mL 0.2623 mL 0.5245 mL 1.3114 mL
    100 mM 0.0420 mL 0.2098 mL 0.4196 mL 1.0491 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    IPTG367-93-1Isopropyl β-D-thiogalactosideBiochemical Assay ReagentsInhibitorinhibitorinhibit

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    Nom du produit:
    IPTG
    Cat. No.:
    HY-15921
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