beta-Hydroxybutyric acid ELISA Kit

Référence OKEH02581

Conditionnement : 96Wells

Marque : Aviva Systems Biology

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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityAll Species
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology beta-Hydroxybutyric acid ELISA Kit (OKEH02581) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-beta-Hydroxybutyric acid antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated beta-Hydroxybutyric acid and incubated. The beta-Hydroxybutyric acid found in the sample or standards competes with the biotinylated beta-Hydroxybutyric acid for limited binding sites on the immobilized anti-beta-Hydroxybutyric acid antibody. Excess unbound biotinylated beta-Hydroxybutyric acid and sample or standard beta-Hydroxybutyric acid is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated beta-Hydroxybutyric acid captured in the well and inversely proportional to the amount of beta-Hydroxybutyric acid which was contained in the sample or standard.
ELISA Kit Range0.156-10umol/L
ELISA Kit RecoveryMean recovery when spiking into Serum and Plasma = 104%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.5% (n = 20)
Mean Inter-assay CV%: < 7.3% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-beta-Hydroxybutyric acid Microplate96 Wells (12 x 8 Well strips)
beta-Hydroxybutyric acid Lyophilized Standard2 x 10 umol
Sample Diluent1 x 20 mL
100X beta-Hydroxybutyric acid-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Additional InformationTarget Abbr: BHA
Target Name: beta-Hydroxybutyric acid
::Pubchem: 441
::Chemical formula: C4H8O3
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma, tissue homogenates, cell culture supernatants and other biological fluids
Sensitivity0.048umol/L
SpecificityNatural and recombinant General beta-Hydroxybutyric acid
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Alias SymbolsBHA, beta-Hydroxybutyric acid, BHA, beta-hydroxybutyrate, 3-hydroxybutyric acid
Protein Namebeta-Hydroxybutyric acid
Description of TargetIn humans, beta-hydroxybutyrate is synthesized in the liver from acetyl-CoA in a reaction catalyzed by the enzyme beta-hydroxybutyrate dehydrogenase and can be used as an energy source by the brain when blood glucose is low. Diabetic patients can have their ketone levels tested via urine or blood to indicate diabetic ketoacidosis. beta-Hydroxybutyric acid (also known as beta-hydroxybutyrate or 3-hydroxybutyric acid) is a ketone body. It is a chiral compound having two enantiomers, D-3-hydroxybutyric acid and L-3-hydroxybutyric acid. Like the other ketone bodies (acetoacetate and acetone), levels of beta-hydroxybutyrate are raised in ketosis.

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Référence
Description
Cond.
Prix HT
MBS1602394-96
 96-Strip-Wells