Histamine ELISA Kit
Référence OKEH02576
Conditionnement : 96Wells
Marque : Aviva Systems Biology
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| Predicted Species Reactivity | All Species | ||||||||||||||||||||||
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| Application | Enzyme-linked Immunosorbent assay-Competitive | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric, OD 450 nm | ||||||||||||||||||||||
| ELISA Kit Duration | ~ 3 Hours | ||||||||||||||||||||||
| ELISA Kit Principle | Aviva Systems Biology Histamine ELISA Kit (OKEH02576) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Histamine antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Histamine and incubated. The Histamine found in the sample or standards competes with the biotinylated Histamine for limited binding sites on the immobilized anti-Histamine antibody. Excess unbound biotinylated Histamine and sample or standard Histamine is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Histamine captured in the well and inversely proportional to the amount of Histamine which was contained in the sample or standard. | ||||||||||||||||||||||
| ELISA Kit Range | 7.8-500ng/mL | ||||||||||||||||||||||
| ELISA Kit Recovery | Mean recovery when spiking into Serum and Plasma = 100% | ||||||||||||||||||||||
| ELISA Kit Reproducibility | Mean Intra-assay CV%: < 4.2% (n = 20) Mean Inter-assay CV%: < 7.3% (n = 20) | ||||||||||||||||||||||
| ELISA Kit Component |
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| Additional Information | Target Abbr: HIS Target Name: Histamine | ||||||||||||||||||||||
| :: | Pubchem: 774 | ||||||||||||||||||||||
| :: | Chemical formula: C5H9N3 | ||||||||||||||||||||||
| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
| Sample Type | Serum, plasma, tissue homogenates, cell culture supernatants and other biological fluids | ||||||||||||||||||||||
| Sensitivity | 3.4 ng/mL | ||||||||||||||||||||||
| Specificity | Natural and recombinant General Histamine | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Competitive ELISA |
| Alias Symbols | HIS, Histamine, HIS |
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| Protein Name | Histamine |
| Description of Target | Histamine is an organic nitrogen compound involved in local immune responses as well as regulating physiological function in the gut and acting as a neurotransmitter. Histamine triggers the inflammatory response. As part of an immune response to foreign pathogens, histamine is produced by basophils and by mast cells found in nearby connective tissues. Histamine increases the permeability of the capillaries to white blood cells and some proteins, to allow them to engage pathogens in the infected tissues. |